GE17-1082-01
Phenyl Sepharose™ High Performance
Cytiva 17-1082-01, pack of 75 mL
Synonym(s):
Phenyl sepharose
About This Item
ligand
phenyl
packaging
pack of 75 mL
manufacturer/tradename
Cytiva 17-1082-01
matrix
6% cross-linked agarose
particle size
24-44 μm
avg. part. size
34 μm
cleaning in place
2-14
working range
3-13
suitability
suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium
General description
Features and Benefits
- Aromatic HIC medium designed for intermediate purification and polishing steps
- Selectivity similar to Phenyl Sepharose™ 6 Fast Flow (low sub) but higher resolution due to the smaller 34 μm bead size
- BioProcess medium supported for industrial applications and well-established in approved processes
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.
This page discusses column packing and preparation techniques for reverse phase chromatography.
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature
Protocols
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.
This page discusses various aspects of sample preparation for chromatographic purification.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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