G8160

Granulocyte Colony-Stimulating Factor from mouse

G-CSF, recombinant, expressed in E. coli, suitable for cell culture

• Synonym(s): G-CSF
• MDL number: MFCD00166475
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: mouse
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 10-60 pg/mL ED₅₀
• quality: endotoxin tested
• mol wt: 19 kDa (179 amino acids including N-terminal methionine)
• packaging: pkg of 5 and 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.01 EU/μg
• UniProt accession no.: P09920
• storage temp.: −20°C
• Gene Information: mouse ... Csf3(12985) , Csf3r(12986)

Description

General description

The Csf3r (colony stimulating factor 3 receptor (granulocyte)) gene encodes a prototypical mobilizing cytokine.

Application

Granulocyte Colony-Stimulating Factor from mouse has been used in cytokine therapy.

Biochem/physiol Actions

G-CSF is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G₀ period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

Granulocyte colony-stimulating factor (G-CSF) is a lineage-restricted hematopoietic growth factor, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors. G-CSF^3,4 is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and mature granulocytes, and promotes differentiation of certain myeloid leukemic cells. Natural G-CSF is a glycoprotein of 177 amino acids and a molecular weight of 19 kDa.⁵ Human and murine G-CSF have about 75% homology and show biological cross-reactivity.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM citric acid, 0.01% TWEEN^® 20, pH 4.0, containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319 - H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves