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G2267

Sigma-Aldrich

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

lyophilized powder, ≥75 units/mg protein

Synonym(s):

D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

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About This Item

CAS Number:
9001-50-7
Enzyme Commission number:
1.2.1.12 (BRENDA, IUBMB)
EC Number:
232-609-4
MDL number:
MFCD00131203
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

rabbit muscle

Quality Level

200

form

lyophilized powder

specific activity

≥75 units/mg protein

composition

Protein, ≥80% biuret

foreign activity

3-Phosphoglyceric phosphokinase ≤0.05%
lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%
triosephosphate isomerase ≤0.1%

shipped in

wet ice

storage temp.

−20°C

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General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.

Application

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
  • for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
  • to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
  • in anti-aggregation assays
  • in purified GAPDH studies

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Unit Definition

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing citrate buffer salts

inhibitor

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Role of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in DNA repair
Kosova A A, et al.
The Journal of Biological Chemistry, 82(6), 643-654 (2017)
Paradoxical inhibition of glycolysis by pioglitazone opposes the mitochondriopathy caused by AIF deficiency
Benit P, et al.
EBioMedicine, 17, 75-87 (2017)
Poly ADP-Ribose Polymerase Inhibition Ameliorates Hind Limb Ischemia Reperfusion Injury in a Murine Model of Type 2 Diabetes
Long C A, et al.
Annals of Surgery, 258(6) (2013)
The C-terminal domain of Escherichia coli trigger factor represents the central module of its chaperone activity
Merz F, et al.
The Journal of Biological Chemistry, 281(42), 31963-31971 (2006)
Oligomerization of a molecular chaperone modulates its activity
Saio T, et al.
eLife, 7(6), e35731-e35731 (2018)
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