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Key Documents

F6765

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Select

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin (typically ≤1 EU/ml)

shipped in

dry ice

storage temp.

−20°C

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Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture. Charcoal stripped FBS is often used instead of FCS to minimize the level of androgen and other hormones provided in 10% serum supplemented media.

Biochem/physiol Actions

Charcoal Stripped Fetal Bovine Serum (FBS) is available for researchers requiring decreased levels of various hormones in their studies. Dextran treated Charcoal is used to selectively remove hormones without nonspecific loss of other serum components. The effectiveness of hormone removal is assessed on the basis of reductions in the levels of T3, T4, and Progesterone pre and post treatment.

This product is designed for researchers involved in studying processes influenced by steroid hormones such as estrogen stimulation and the obesity process and other areas that benefit from reduced hormone levels such as certain types of viral infections.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

The product is not available in Australia. Please view our protocol for Charcoal-stripping FBS to deplete hormones here.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Ahmad Sharanek et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(12), 1292-1303 (2017-09-21)
Drug-induced intrahepatic cholestasis is characterized by cellular accumulation of bile acids (BAs), whose mechanisms remain poorly understood. The present study aimed to analyze early and progressive alterations of BA profiles induced by cyclosporine A, chlorpromazine, troglitazone, tolcapone, trovafloxacin, and tacrolimus
Christian D. Hernandez-Silva et al.
Anti-cancer agents in medicinal chemistry, 19(6), 783-791 (2019-02-08)
The effect of estrogen has been traditionally studied through the modulation of its alpha and beta nuclear receptors; however, the G Protein-Coupled Estrogen Receptor (GPER) has been recently involved in the pathology of numerous tumors. Although the study of GPER
Jonathan T Lei et al.
Cell reports, 24(6), 1434-1444 (2018-08-09)
RNA sequencing (RNA-seq) detects estrogen receptor alpha gene (ESR1) fusion transcripts in estrogen receptor-positive (ER+) breast cancer, but their role in disease pathogenesis remains unclear. We examined multiple ESR1 fusions and found that two, both identified in advanced endocrine treatment-resistant
Anna K Wójtowicz et al.
Neurotoxicity research, 35(1), 183-195 (2018-08-19)
Di-(2-ethylhexyl) phthalate (DEHP) is used as a plasticizer in various plastic compounds, such as polyvinyl chloride (PVC), and products including baby toys, packaging films and sheets, medical tubing, and blood storage bags. Epidemiological data suggest that phthalates increase the risk
Yusuke Ohnishi et al.
PloS one, 3(5), e2248-e2248 (2008-05-22)
Allele-specific gene silencing by RNA interference (RNAi) is therapeutically useful for specifically inhibiting the expression of disease-associated alleles without suppressing the expression of corresponding wild-type alleles. To realize such allele-specific RNAi (ASP-RNAi), the design and assessment of small interfering RNA
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Articles

Choosing the optimal cell culture media for vaccine production

Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS).

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