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F4648

Sigma-Aldrich

Fast Red TR/Naphthol AS-MX

SIGMAFAST™, fluorogenic, tablet

Synonym(s):

4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets

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About This Item

MDL number:
MFCD00132253
UNSPSC Code:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 1 mL

Quality Level

200

form

tablet

solubility

water: 1 tablet/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

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Application

SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used:
  • in alkaline phosphatase staining in organ sections
  • for staining chondrogenic spheroids in immunohistochemistry studies
  • for the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections

Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.

Reconstitution

Each tablet dissolved in 1 mL deionized water yields a ready-to-use buffered solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H314,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Christina Anna Stratopoulou et al.
Journal of clinical medicine, 10(19) (2021-10-14)
Increasing imaging data point to a link between deep endometriotic nodules (DENs) and uterine adenomyosis (AD). The study aimed to investigate this link at the histological level and detect potential features shared by the two diseases. We collected formalin-fixed paraffin-embedded
Telomere length and telomerase activity during expansion and differentiation of human mesenchymal stem cells and chondrocytes
Parsch D, et al.
Journal of Molecular Medicine, 82(1), 49-55 (2004)
PTD-mediated loading of tumor-seeking lymphocytes with prodrug-activating enzymes
Yang Q, et al.
The AAPS Journal, 10(4), 614-614 (2008)
Plasticity of endothelial cells: rapid dedifferentiation of freshly isolated high endothelial venule endothelial cells outside the lymphoid tissue microenvironment
Lacorre DA, et al.
Blood, 103(11), 4164-4172 (2004)
Kathryn B Moore et al.
Developmental biology, 437(1), 27-40 (2018-03-09)
Neural basic helix-loop helix (bHLH) transcription factors promote progenitor cell differentiation by activation of downstream target genes that coordinate neuronal differentiation. Here we characterize a neural bHLH target gene in Xenopus laevis, vexin (vxn; previously sbt1), that is homologous to
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Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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