D8899
Deoxyribonucleic acid, single stranded from calf thymus
lyophilized powder
Synonym(s):
DNA single stranded
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
Recommended Products
grade
for molecular biology
Quality Level
form
lyophilized powder
mol wt
~50 kb
composition
ssDNA, ≥65%
storage temp.
−20°C
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General description
High quality single-stranded template DNA isolated from the thymus of male and female calves.
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Features and Benefits
• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
Quantity
One mg DNA is approx. 25 A260 units.
Preparation Note
Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.
Reconstitution
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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