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CMC0004

Sigma-Aldrich

SIG10 MAX Electrocompetent Cells

Escherichia coli, rod shaped

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About This Item

UNSPSC Code:
41106202
NACRES:
NA.85

product name

SIG10 MAX Electrocompetent Cells, for general cloning & library production

biological source

Escherichia coli

grade

for molecular biology

form

buffered aqueous suspension

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥2 x 1010 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The SIG10 MAX Electrocompetent Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

  • share the most useful genetic elements of standard cloning strains like DH5a, DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
  • incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability.
  • provide solutions for a wide range of applications at economical prices.
  • Blue - white screening

Components

  • SIG10 MAX electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

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Articles

Bacterial Transformation

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.

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