Skip to Content
Merck
All Photos(1)

Key Documents

OE19 Cell Line human

96071721, human esophagus, Epithelial

Synonym(s):

JROECL19, JROECL19 Cells, OE-19 Cells, OEC19 Cells

Sign Into View Organizational & Contract Pricing

Select a Size


Select a Size

Change View

About This Item

UNSPSC Code:
41106514

product name

OE19 Cell Line human, 96071721

biological source

human esophagus

growth mode

Adherent

karyotype

Aneuploid

morphology

Epithelial

products

Not specified

receptors

MHC class I

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Cell Line Origin

Human Caucasian oesophageal carcinoma

Cell Line Description

The cell line OE19, also known as JROECL19, was established in 1993 from an adenocarcinoma of gastric cardia/oesophageal gastric junction of a 72 year old male patient. The tumour was identified as pathological stage III (UICC) and showed moderate differentiation. The cell line OE19 expresses HLA-A, -B and -C antigens (MHC class I) constitutively. Treatment with interferon-gamma induced the expression of ICAM-1 (CD54). Expression of HLA-DR (MHC class II) on interferon-gamma addition was only measured in a sub-population of OE19. The cells express epithelial cytokeratins and are tumourigenic in nude mice.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

OE19 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells.[1] It has also been used to study microRNA-330-5p as a modulator of neoadjuvant chemoradiotherapy sensitivity.[2]
Study of oesophageal cancer

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,13
D13S317: 9,11
D16S539: 12,13
D5S818: 11,14
D7S820: 8
THO1: 8,9
TPOX: 8
vWA: 16,17

Culture Medium

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8, i.e., seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. These cells grow slowly in islands and when resuscitated from frozen it can take up to 7 days to reach 70% confluence.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service