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Key Documents

MLg

90112707, mouse lung, Fibroblast-like

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About This Item

UNSPSC Code:
41106514

product name

MLg, 90112707

biological source

mouse lung

growth mode

Adherent

karyotype

Modal no. 64; hypodiploid

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse ddY lung

Cell Line Description

Established from normal lung tissue of a new-born, ddY strain mouse. Both induced and spontaneous release of C-type particles have been reported.

Application

Virus studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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