OX-24

Reactivity: Anti-Factor H, human, 00010403

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myeloma: NS0
• Reactivity: Anti-Factor H, human
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse x Mouse hybridoma

Cell Line Description

The hybridoma line OX-24 was developed by immunizing BALB/c mice with Factor H followed by fusion of spleen cells with NS0 mouse myeloma cells. The antibody was shown to detect the human complement control protein, Factor H. OX-24, which is also known as MRC-OX24 was reported to increase cofactor activity of Factor H in Factor I-mediated cleavage of soluble C3b. The antibody inhibited the binding of radioactive-labelled Factor H to surface-bound C3b. OX-23 (Sigma Catalogue number. 00010402) was shown to decrease this inhibition.

Immunogen

Human Factor H

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 10% FBS / FCS (F2442)

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.