C9281
Cholesterol Esterase from Pseudomonas fluorescens
lyophilized powder, ≥10,000 units/g protein
Synonym(s):
CE, bile salt-stimulated lipase, cholesteryl ester hydrolase, pancreatic cholesterol esterase, Sterol-ester acylhydrolase
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About This Item
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biological source
Pseudomonas fluorescens
Quality Level
Assay
10-30% (TCA-Biuret)
form
lyophilized powder
specific activity
≥10,000 units/g protein
mol wt
~129 kDa
composition
Protein, ~20%
storage condition
under inert gas (argon)
technique(s)
cell based assay: suitable
color
tan to brown
pH
7-9
solubility
0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
storage temp.
−20°C
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General description
Research area: Cell Signaling
Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).
Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).
Application
Cholesterol esterase from Pseudomonas fluorescens has been used in:
- in cholesterol esterase assay to quantify total cholesterol from human blood serum samples
- a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations
- an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase
- for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs)
- to hydrolyze native cholesterol ester (CE) during filipin staining for detection of CE within the retinal frozen sections
This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.
Biochem/physiol Actions
Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis..
Other Notes
Contains potassium phosphate and TRITON® X-100.
Unit Definition
One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.
Analysis Note
Protein determined by biuret.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Articles
Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.
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