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C8890

Sigma-Aldrich

4-Chloro-1-naphthol

horseradish peroxidase substrate, fluorogenic, ≥98% (GC), crystalline

Synonym(s):

4-chloronaphthalen-1-ol

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About This Item

Linear Formula:
ClC10H6OH
CAS Number:
604-44-4
Molecular Weight:
178.61
Beilstein:
509750
EC Number:
210-068-5
MDL number:
MFCD00003974
UNSPSC Code:
12352204
PubChem Substance ID:
57654054
NACRES:
NA.83

product name

4-Chloro-1-naphthol, crystalline

Assay

≥98% (GC)

Quality Level

200

form

crystalline

mp

118-121 °C (lit.)

solubility

acetone: 50 mg/mL, clear, colorless to very faintly brown

storage temp.

−20°C

SMILES string

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI key

LVSPDZAGCBEQAV-UHFFFAOYSA-N

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Application

4-Chloro-1-naphthol has been used to visualize protein bands in western blotting. It has also been used in staining polyvinylidene fluoride (PVDF) membrane by Nakane method.

Biochem/physiol Actions

4-Chloro-1-naphthol serves as a substrate for horseradish peroxidase.

Substrates

Chromogenic peroxidase substrate useful in enzyme-linked detection procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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