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C7902

Sigma-Aldrich

Calcium chloride dihydrate

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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About This Item

Empirical Formula (Hill Notation):
CaCl2 · 2H2O
CAS Number:
10035-04-8
Molecular Weight:
147.01
EC Number:
233-140-8
MDL number:
MFCD00149613
UNSPSC Code:
12352302
PubChem Substance ID:
24892994
NACRES:
NA.71

product line

BioReagent

Quality Level

200

Assay

≥99.0%

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

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General description

Calcium chloride (CaCl2) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl2 is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl2 is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl2 along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl2 on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.

Application

Calcium chloride dihydrate has been used:
  • in the formulation of media
  • in sperm collection and preparation
  • in cell culture and protoplast preparation

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes
PANCARCI S M, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16(5) (2010)
Hemolymph analysis and evaluation of newly formulated media for culture of shrimp cells (Penaeus stylirostris)
Shimizu C, et al.
In Vitro Cellular & Developmental Biology. Animal, 37(6), 322-329 (2001)
Marco Wallenborn et al.
PloS one, 15(7), e0234986-e0234986 (2020-07-08)
Glioblastoma is a common, malignant brain tumor whose disease incidence increases with age. Glioblastoma stem-like cells (GSCs) are thought to contribute to cancer therapy resistance and to be responsible for tumor initiation, maintenance, and recurrence. This study utilizes both SNP
Biomolecule delivery into canola protoplasts by centrifuging cells with microbubbles
He C, et al.
Febs Letters, 587(3), 285-290 (2013)
Grace C Lin et al.
Tissue barriers, 8(2), 1748459-1748459 (2020-04-22)
During the last years, the popularity of saliva has been increasing for its applicability as a diagnostic fluid. Blood biomarker molecules have to cross the blood-saliva barrier (BSB) in order to appear in saliva. The BSB consists of all oral
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Articles

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Protocols

RNAi In Situ

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition

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