C7642
Concanavalin A from Canavalia ensiformis (Jack bean)
FITC conjugate, Type IV, lyophilized powder
Synonym(s):
ConA
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
biological source
Canavalia ensiformis
Quality Level
conjugate
FITC conjugate
type
Type IV
form
lyophilized powder
composition
Protein, 5-15% biuret
extent of labeling
3-6 mol FITC per mol protein
impurities
Carbohydrate, essentially free
storage temp.
−20°C
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Application
For the fluorescent detection of glycoproteins containing α−D-mannose or α−D-glucose
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Packaging
Package size based on protein content
Physical form
Lyophilized powder containing phosphate buffer salts and NaCl
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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