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C6366

SAFC

EX-CELL® CHO Cloning Medium

Animal-component free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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About This Item

UNSPSC Code:
12352207

Quality Level

sterility

sterile-filtered

product line

EX-CELL®

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

General description

Developed to meet the needs of the biotechnology industry, this animal component-free medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free suspension culture.

Application

This animal component-free medium is designed to support clonal survival and growth of Chinese Hamster Ovary (CHO) cell lines, with results comparable to the traditional method using 10% fetal bovine serum. Aseptically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

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replaced by

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992).

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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