C2571
Anti-Human IgG (Fc specific)-Cy3 antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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About This Item
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biological source
goat
Quality Level
conjugate
CY3 conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Related Categories
Application
Anti-Human IgG (Fc specific)-Cy3 antibody produced in goat was used to screen sera on glycopeptide microarrays at a dilution of 1:1000. It was used at a working dilution of 1:100 for immunohistochemistry analysis of mouse footpad sections incubated with human polyclonal anti-Leishmania serum.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Human IgG (Fc specific) antibody allows for the visualization of proteins by fluorescent microscopy.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Legal Information
Cy is distributed under license from Amersham Biosciences Limited.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Journal of immunological methods, 432, 57-64 (2016-02-24)
Enzyme-linked immunosorbent assays (ELISAs) have traditionally been used to detect alloantibodies in patient plasma samples post hematopoietic cell transplantation (HCT); however, protein microarrays have the potential to be multiplexed, more sensitive, and higher throughput than ELISAs. Here, we describe the
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