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C1416

Sigma-Aldrich

Chu (N6) Basal Salt Mixture

powder, suitable for plant cell culture

Synonym(s):

Nutrient Solution

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

form

powder

Quality Level

200

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

Application

Chu (N6) Basal Salt Mixture has been used:
  • as medium for Zea mays embryo culture
  • as a component of zygotic medium for culturing rice zygotes
  • as a medium for onion cells epidermal layer for bombardment based transformation studies

Chu (N6) Basal Salt Mixture is composed of macro- and micronutrients as described by Chu . Chu (N6) Basal Salt Mixture may be used as a chemstock to support the reconstitution of Chu (N6) medium.

Formula variant

With the macro- and micronutrients as described by Chu (1975, 1981).

Media Formulation

Quantity

Formulated to contain 4.0 grams of powder per liter of medium.

Pictograms

Flame over circle
GHS03

Signal Word

Warning

Hazard Statements

H272

Hazard Classifications

Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The N6 medium and its application to anther culture of cereal crops, in Plant Tissue Culture.
Chu, C.C.
Plant Tissue Culture. Proceedings of the Peking Symposium, 43-50 (1981)
Establishment of an efficient medium for anther culture of rice, through comparative experiments on the nitrogen sources.
Chu, C.C., et al.
Scientia Sinica, 18, 659-668 (1975)
Q Zhang et al.
Plant cell reports, 23(9), 587-595 (2005-02-03)
Somatic embryos directly formed at cut edges or on the surface of leaf explants, around cut ends or along side surfaces of petiole and stem explants of 'Golden Pothos' [Epipremnum aureum (Linden & Andre) Bunt.] on Murashige and Skoog (MS)
P Thobunluepop et al.
Pakistan journal of biological sciences : PJBS, 12(15), 1085-1089 (2009-12-01)
Somatic embryogenesis in sweet corn has been reported by a number of workers. However, the knowledge maintaining storage life, vigor and viability of these somatic embryos are limited. A model system of synchronous somatic embryos production combined with encapsulation to
Electro-fusion of gametes and subsequent culture of zygotes in rice
Toda E, et al.
Bio-protocol, 6, e2074-e2074 (2016)
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Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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