A9544

Anti-Human IgG (Fc specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti Human Igg, Anti-Human IgG Antibody - Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat, Anti-Human Igg, anti human IgG antibody
• MDL number: MFCD00162437
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 300
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct ELISA: 1:55,000; dot blot: 1:40,000 (chemiluminescent); immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is specific for human IgG and human IgG, Fc fragment. The product does not react with human IgG, Fab fragment, light chains, IgA, and IgM. Additionally, the antibody does not bind to mouse and rat IgG. Goat anti-human IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.

Specificity

Specificity of the Anti-Human IgG is determined prior to conjugation by ELISA. The conjugate is specific for human IgG and human IgG, Fc fragment. Cross reactivity of the antibody-conjugate is also determined by ELISA. The conjugate shows no reactivity with human IgG, Fab fragment, human light chains, human IgA, human IgM or with mouse IgG and rat IgG.
Binds human IgG; does not bind other human Igs.
Provides reduced background with mouse or rat samples.

Immunogen

Human IgG, Fc fragment

Application

A modified monoclonal antibody specific immobilization of platelet antigens (MAIPA) assay was performed on human platelet eluates using alkaline phosphatase-conjugated goat anti-human, Fc specific, antibody as the secondary with a 30 minute incubation.

Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA and western blot at 1:5000 dilutions. The product can also be used for dot blot (1:40,000) and immunohistochemistry (1:150 using formalin-fixed, paraffin-embedded sections).

Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody has been used in

• quantitative sandwich ELISA
• α-GalC ELISA
• ELISA assays
• ELISAs for IgG subclasses

Biochem/physiol Actions

Immunoglobulin G (IgG) regulates immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Immunoglobulin G (IgG) mainly participates in hypersensitivity type II and type III.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% BSA, 1 mM MgCl₂ and 15 mM sodium azide

Storage and Stability

Store at 2-8 °C. Do Not Freeze.

Other Notes

No cross-reaction with mouse and rat IgG.
Working dilutions should be determined by titration assay. Due to differences in assay systems, these titers may not reflect the user′s actual working dilution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable