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46947

Sigma-Aldrich

Fluorescein isothiocyanate – Dextran 500000-Conjugate

(FITC:Glucose = 1:100)

Synonym(s):

Dextran 500′000 Fluorescein isothiocyanate Conjugate

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

form

powder or crystals

Quality Level

100

composition

(FITC:Glucose = 1:100)

extent of labeling

0.01 mol/mol FITC glucose (substitution)

technique(s)

permeability assay: suitable

solubility

H2O: 25 mg/mL, clear to very faintly turbid, yellow to orange

fluorescence

λex 490 nm; λem 520 nm (pH 8.0)

storage temp.

2-8°C

Application

Fluorescein isothiocyanate – Dextran 500000-Conjugate is used as a fluorescent tissue permeability assay reagent and as a diffusion probe for evaluation of biomaterials such as hydrogels and lipid vesicles.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Lisa M Dominak et al.
Langmuir : the ACS journal of surfaces and colloids, 24(23), 13565-13571 (2008-11-05)
We report the effect of macromolecular crowding on encapsulation efficiency of fluorescently labeled poly(ethylene glycol) (PEG) and dextran polymers within individual giant lipid vesicles (GVs). Low concentrations of the fluorescently labeled polymers (82 nM to 186 pM) were mixed with
Tina Vermonden et al.
Macromolecules, 43(2), 782-789 (2010-10-05)
Hydrogel formation triggered by a change in temperature is an attractive mechanism for in situ gelling biomaterials for pharmaceutical applications such as the delivery of therapeutic proteins. In this study, hydrogels were prepared from ABA triblock polymers having thermosensitive poly(N-(2-hydroxypropyl)
Ayesha Chaudhary et al.
Biotechnology and bioengineering, 104(6), 1075-1085 (2009-08-06)
The feasibility of dissolved-core alginate-templated fluorescent microspheres as "smart tattoo" glucose biosensors was investigated in simulated interstitial fluid (SIF). The sensor works on the principle of competitive binding and fluorescence resonance energy transfer. The sensor consists of multilayer thin film
Joel Hagman et al.
Biomacromolecules, 11(12), 3359-3366 (2010-11-09)
The time-dependent diffusion and mechanical properties of gelatin in solution, in the gel state, and during the sol/gel transition were determined using fluorescence recovery after photobleaching (FRAP) and rheology. The parameters in the experimental design were 2% w/w and 5%
Ziwei Fu et al.
American journal of physiology. Renal physiology, 316(3), F530-F538 (2018-11-01)
The (pro)renin receptor (PRR) is a new component of the renin-angiotensin-aldosterone system (RAAS) and regulates renin activity. The objective of the present study was to test potential roles of the renal PRR and intrarenal RAAS in the physiological status of

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