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Key Documents

90107

Sigma-Aldrich

Gram′s iodine solution

for microscopy

Synonym(s):

Potassium Triiodide

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About This Item

UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

Quality Level

100

product line

BioChemika

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

color

brown to very dark brown

refractive index

n20/D 1.335

density

1.005 g/mL at 20 °C

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

bacteria

Related Categories

Application

Gram’s iodine solution is used as a mordant in the Gram staining process. It is added after the primary staining step with Crystal Violet; the resultant Crystal Violet - Iodine complex imparts purple color to the cells.

Biochem/physiol Actions

Iodine(I) interacts with Crystal Violet(CV) to form large CV-I complexes within the cytoplasm and outer layers of the cell.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG6997
BCCG6999
BCCG6998
BCCG7001
BCCG7000

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Practical Methods for Environmental Microbiology and Biotechnology (2002)

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