114413

Bromothymol Blue

ACS reagent, Dye content 95 %

• Synonym(s): 3′,3″-Dibromothymolsulfonphthalein
• Empirical Formula (Hill Notation): C₂₇H₂₈Br₂O₅S
• CAS Number: 76-59-5
• Molecular Weight: 624.38
• Beilstein: 373934
• EC Number: 200-971-2
• MDL number: MFCD00005872
• UNSPSC Code: 12171500
• PubChem Substance ID: 24847171
• NACRES: NA.47

Properties

• grade: ACS reagent
• Quality Level: 200
• form: powder
• composition: Dye content, 95%
• color: pink-purple
• visual transition interval: 6.0-7.6, yellow to blue(passes test)
• mp: 200-202 °C ((392 - 396 °F) - lit.); 200-202 °C (lit.)
• λmax: 420 nm
• ε (extinction coefficient): ≥17000 at 419-425 nm in methanol; ≥8000 at 326-332 nm in methanol; ≥9000 at 279-285 nm in methanol
• storage temp.: room temp
• SMILES string: CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C
• InChI: 1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3
• InChI key: NUHCTOLBWMJMLX-UHFFFAOYSA-N

Description

General description

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Application

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:

• as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
• for the demonstration of fungal hyphae within plant roots
• in the assessment of the soluble acidity within single rice grains
• use in an agar gel medium for enumeration of Bacillus cereus
• as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves