HKG6PDH-RO
Roche
Hexokinase/Glucose-6-Phosphate Dehydrogenase (HK/G6P-DH)
from yeast/Leuconostoc, overproducer
Synonym(s):
G6P-DH, Glucose-6-Phosphate DehydrogenaseHK, Hexokinase
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About This Item
UNSPSC Code:
12352204
Recommended Products
biological source
yeast
Quality Level
form
suspension
mol wt
dimer 110,000 Da
packaging
pkg of 10 mL (10737275001 [30 mg])
pkg of 5 mL (10127825001 [15 mg])
manufacturer/tradename
Roche
concentration
3.21 mg/mL
technique(s)
activity assay: suitable
color
white
pH
5.5-6.5
solubility
water: miscible
suitability
suitable for UV spectrophotometry and general use
application(s)
life science and biopharma
storage temp.
2-8°C
General description
Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.
Application
Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.
Components
EC 2.7.1.1 & 1.1.1.49
Unit Definition
One unit (U) HK will phosphorylate 1 μmol of D-glucose in one minute at +25 °C and pH 7.6.
One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated.
Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate
One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated.
Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.
Other Notes
For life science research only. Not for use in diagnostic prodcedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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