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UFC201024

Millipore

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa

Synonym(s):

Centrifugal concentrator, 10 kDa MWCO, 2 mL sample volume

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

material

polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing

Quality Level

400

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

parameter

2 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

119.7 mm

diam.

15.9 mm

filtration area

1 cm2

volume

2 mL

pore size

10 kDa MWCO

shipped in

ambient

General description

Amicon® Ultra-2 centrifugal filter devices offer fast ultrafiltration. It has the potential to recover high concentration factors and easy concentrate from dilute and complex sample matrices. Amicon® Ultra-2 devices are supplied non-sterile and are for single use only.

Application

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates.
  • Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.
Amicon® Ultra-2 Centrifugal Filter Unit has been used:
  • to reduce large volume biofluids prior to extracellular vesicle (EV) isolation or to concentrate EVs
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and purified samples
  • Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular labels from a reaction mix, and protein removal before high-performance liquid chromatography (HPLC)
  • Desalting, buffer exchange, or diafiltration

Features and Benefits

  • Amicon® Ultra 2ml 10K 24PK
  • Concentrates 2 mL down to 15-70 μl
  • 98% sample recovery
  • Fast processing time - 10 to 30 minute spin times
  • Engineered dead stop
  • The reverse spin capability provides consistent recoveries

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 2 mL down to 15-70 μL
  • 98% sample recovery
  • Fast processing time: 10 to 30-minute spin times
  • Engineered dead stop
  • Reverse spin capability provides consistent recoveries

Linkage

Replaces: 4205

Other Notes

The Amicon® Ultra-2 device is supplied with two tubes. During operation, one tube is used to collect filtrate; the other to cap the device during concentration and subsequently to recover the concentrated sample. Amicon Ultra-2 Centrifugal filter devices are for research use only and not for use in diagnostic procedures.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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Bao Quoc Tran et al.
Data in brief, 6, 68-76 (2016-01-23)
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated

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Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.

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