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Key Documents

ST1673

Sigma-Aldrich

Anti-AFP Mouse mAb (1G7)

liquid, clone 1G7, Calbiochem®

Synonym(s):

Anti-α-Fetoprotein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G7, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AFP(174)

General description

Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.
Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
Recognizes the ~70-75 kDa AFP protein in HepG2 cells.

Immunogen

A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein

Application

ELISA (see coments)

Immunoblotting (1-5 µg/ml)

Immunocytochemistry (10 µg/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells
Positive Control
HepG2 cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Raymond C B Wong et al.
Stem cell research, 22, 13-15 (2017-09-28)
We report the generation of the hiPSC line CERAi001-A-6 from primary human dermal fibroblasts. Reprogramming was performed using episomal vector delivery of OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA for p53.
Kagistia Hana Utami et al.
Biological psychiatry, 88(6), 500-511 (2020-07-13)
Fragile X syndrome (FXS) is a neurodevelopmental disorder caused by epigenetic silencing of FMR1 and loss of FMRP expression. Efforts to understand the molecular underpinnings of the disease have been largely performed in rodent or nonisogenic settings. A detailed examination

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