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PC408

Sigma-Aldrich

Anti-Green Fluorescent Protein (26-39) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-GFP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.

Recognizes the ~28 kDa GFP and some GFP fusion proteins.

Immunogen

Aequora victoria
a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein

Application

Immunoblotting (1:2000)

Immunocytochemistry (1:200)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 1 mg/ml BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.
This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kathryn A Tworkowski et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(16), 6103-6108 (2008-04-17)
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