MABT78

Anti-mouse CD44 (H-CAM) Antibody, clone KM201

clone KM201, from rat

• Synonym(s): CD44 antigen (homing function and Indian blood group system), CDw44 antigen, HCAM, H-CAM, HUTCH-1, Phagocytic glycoprotein I, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, Heparan sulfate proteoglycan, Hermes antigen, Hyalu
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: KM201, monoclonal
• species reactivity: mouse
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001034240
• UniProt accession no.: P15379
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CD44(960)

Description

General description

CD44, also known as H-CAM is a distinct adhesion molecule that, with its 13 known isoforms, comprises a family of transmembrane glycoproteins involved in a wide variety of significant biological processes. Members belonging to this family are involved in apoptosis, migration, metastasis, tumor progression, lymphocyte homing, and the attachment of hematopoietic cells to stromal cells. CD44 is the primary cell surface receptor for hyaluronic acid (HA), and is also a notable target for WNT signaling within the intestinal mucosa. Recent studies have used CD44 marker as a means to isolate different cancer stem cells (CSC) such as; colorectal CSC, pancreatic CSC, and prostate CSC.

Specificity

The antibody recognizes CD44 near the hyaluronate binding domain.

Immunogen

Epitope: Near the hyaluronate binding domain

Mouse bone marrow-derived stromal cells

Application

Detect mouse CD44 (H-CAM) using this Anti-mouse CD44 (H-CAM) Antibody, clone KM201 validated for use in FC.

Research Category
Cell Structure

Stem Cell Research

Research Sub Category
Adhesion (CAMs)

Hematopoietic Stem Cells

Quality

Evaluated by Flow Cytometry in mouse bone marrow cells.

Flow Cytometry Analysis: 0.1 µg of this antibody detected CD44 in mouse bone marrow cells.

Target description

86 kDa calculated

Linkage

Replaces: CBL1308

Physical form

Format: Purified

Protein G

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse bone marrow cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable