MABT368

Anti-Troponin T (Cardiac Muscle) Antibody, clone 9C2.1

clone 9C2.1, from mouse

• Synonym(s): Troponin T, cardiac muscle, Cardiac muscle troponin T, cTnT, TnTc
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9C2.1, monoclonal
• species reactivity: mouse, rat
• species reactivity (predicted by homology): human (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_000355
• UniProt accession no.: P45379
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TNNT2(7139)

Description

General description

Troponin T, cardiac muscle (UniProt P45379; also known as Cardiac muscle troponin T, cTnT, TnTc) is encoded by the TNNT2 (also known as CMD1D, CMH2, RCM3) gene (Gene ID 7139) in human. Troponin is a complex composed of three regulatory proteins, troponin C, troponin I, and troponin T). Troponin is found in both skeletal muscle and cardiac muscle where it plays an integral role in muscle contraction. Troponin T (TnT) is the component that binds tropomyosin, while troponin I (TnI) binds actin in thin myofilaments to hold the actin-tropomyosin complex in place. Three tissue TnT types exist, the slow skeletal troponin T1 encoded by the TNNT1 gene, the cardiac troponin T2 encoded by the TNNT2 gene, and the fast skeletal troponin T3 encoded by the TNNT3 gene.

Specificity

Expected to react with human troponin T spliced isoforms 1-8 and 10, as well as all four of mouse troponin T spliced isoforms (Embryonic, A1, A2, and A3B isoforms; UniProt P50752) and both of rat troponin T spliced isoforms (Embryonic and Adult isoforms; UniProt P50753)

Immunogen

Epitope: Internal.

Recombinant human cardiac muscle troponin T internal fragment.

Application

Detect Troponin T using this Anti-Troponin T (Cardiac Muscle) Antibody, clone 9C2.1 validated for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Troponin T (Cardiac Muscle) in rat cardiac muscle tissue.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Troponin T (Cardiac Muscle) in 10 µg of mouse heart tissue lysate.

Target description

~36 kDa observed. 35.8 kDa (isoform Embryonic), 35.0 kDa (isoform A1), 34.5 kDa (isoform A2), and 34.2 kDa (Isoform A3B) of mouse cardiac muscle troponin T spliced isoforms calculated.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable