M04S-03-5PK
CellASIC ONIX switching plate mammalian cells (4 chamber)
The M04 switching plates enable continuous perfusion culture for live cell analysis. Laminar flow provides rapid & uniform solution exchange for maximum cell health. The glass coverslide bottom surface ensures the highest resolution optical viewing.
Synonym(s):
CellASIC ONIX Plate, Mammalian Cell Plate, ONIX Switching Plate
About This Item
Recommended Products
material
PMMA
glass
polycarbonate
polydimethylsiloxane (PDMS)
manufacturer/tradename
CellASIC® ONIX
W × L × H
85.48 mm × 127.76 mm × 14.35 mm
compatibility
for use with cells sized up to 50.0 μm
shipped in
ambient
General description
Application
- in live-cell imaging to study the contribution of phi-particle in the autoinhibition of dynein and the activation of dynein by dynactin
- in live-cell imaging of fcSAgA binding and immunoglobulin M surface clustering by fluorescence microscopy
- in multiple epidermal growth factor (EGF)pulse experiments
- time-lapse analysis of adherent cells
- long-term continuous perfusion experiments (3 days typical)
- solution exchange experiments (induction, inhibition, drug dosing, etc.)
- automated immunostaining and on-demand fixation of live cells within the culture chamber
- comparison of up to four different cell types or exposure conditions (media components) in parallel, temperature, and gas atmospheric control (temperature shift, anoxic conditions, etc.)
Features and Benefits
- Compatible with CellASIC® ONIX2 Microfluidic system and ONIX2 Manifolds
- Enables perfusion-based, long-term, live-cell analysis with solution switching
- The plate offers a controlled and dynamic microenvironment for 2D and 3D cell cultures
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
This page describes novel microfluidic approaches to trap two different cell types in precise locations in order to image and study their interactions over time.
This page describes a novel, optimized microfluidic platform for long-term culture of primary neurons to monitor dynamic cellular processes in real time.
Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.
This page shows the long-term culture and in-plate staining protocols using the CellASIC ONIX Microfluidic platform.
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Convert your microscope to a live cell imaging system – the CellASIC® ONIX2 system allows precise manipulation of the cell culture environment for dynamic live cell imaging and analysis using mammalian, yeast, and bacterial cells.
Convert your microscope to a live cell imaging system – the CellASIC® ONIX2 system allows precise manipulation of the cell culture environment for dynamic live cell imaging and analysis using mammalian, yeast, and bacterial cells.
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