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ISEQ85R

Millipore

Immobilon®-PSQ Membrane

8.5 cm x 10 m roll, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane

Synonym(s):

Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031613
NACRES:
NB.23

product name

Immobilon®-PSQ Membrane, PVDF, 0.2 m, 8.5 cm x 10 m roll, 0.2 um filter ( 0.2 micron ) pore size Hydrophobic PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

material

PVDF membrane
plain filter
white filter

Quality Level

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

8.5 cm × 10 m

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin)
340 μg/cm2 adsorption capacity (BSA)
448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
radioactive

shipped in

ambient

General description

The Immobilon®-PSQ membrane is a 0.2 μm microporous polyvinylidene fluoride (PVDF) membrane. It is for protein sequencing and immunodetection of proteins with molecular weights of less than 20,000 Daltons.
Filter Code: ISEQ
Primary Binding Mechanisms: Electrostatic, hydrophobic

Application

Immobilon®-PSQ Membrane, PVDF, 0.2 μm, 8.5 cm x 10 m roll has been used in western blot analysis.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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Dattatraya Gautam Rawale et al.
Chemical science, 12(19), 6732-6736 (2021-05-28)
The conservation of chemoselectivity becomes invalid for multiple electrophilic warheads during protein bioconjugation. Consequently, it leads to unpredictable heterogeneous labeling of proteins. Here, we report that a linchpin can create a unique chemical space to enable site-selectivity for histidine and

Protocols

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits

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