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CP45

Sigma-Aldrich

Anti-Clathrin Heavy Chain Mouse mAb (X22)

liquid, clone X22, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

X22, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

bovine, mouse, rat, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CLTCL1(8218)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 mouse myeloma cells. Recognizes the ~180 kDa clathrin heavy chain protein.
Recognizes the ~180 kDa clathrin heavy chain protein.
This Anti-Clathrin Heavy Chain Mouse mAb (X22) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Clathrin Heavy Chain.

Immunogen

Human
clathrin heavy chain purified from human brain

Application

Immunoblotting (1:500)

Immunofluorescence (1:1000, indirect)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Bauerfeind, R., et al. 1996. J. Neurocytol.25, 701.
Zhang, J.Z., et al. 1994. Cell78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol.122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol.122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol.6, 151.
Brodsky, F.M. 1985. J. Cell Biol.101, 2047.
Immunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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April Thelemann et al.
Molecular & cellular proteomics : MCP, 4(4), 356-376 (2005-01-20)
Overexpression and enhanced activation of the epidermal growth factor (EGF) receptor are frequent events in human cancers that correlate with poor prognosis. Anti-phosphotyrosine and anti-EGFr affinity chromatography, isotope-coded muLC-MS/MS, and immunoblot methods were combined to describe and measure signaling networks

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