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Key Documents

AB947

Sigma-Aldrich

Anti-Apolipoprotein E Antibody

serum, Chemicon®

Synonym(s):

ApoE

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

goat

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

primate, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

Specificity

Monospecific for Apolipoprotein E by immunoelectrophoresis when tested against pooled human plasma and twice concentrated pooled human serum. Reacts with Apo E isoforms E2, E3 and E4 by Western blot.

Immunogen

Recombinant human apolipoprotein E.

Application

Detect Apolipoprotein E using this Anti-Apolipoprotein E Antibody validated for use in IH & WB.
Research Category
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
Western blot (Schwab, 1996): 1:1,000-1:2,000

Immunohistochemistry (Summers, 1998; Wisniewski & Frangione, 1992; Schwab, 1996)

Optimal working dilutions must be determined by the end user.

Target description

34 kDa

Physical form

Delipidated goat antiSerum, 0.2 μm filtered. Liquid in Tris-saline, pH 7.4 with 0.1% sodium azide as preservative.
Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Liver tissue

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Apolipoprotein E (ApoE) isoform-dependent lipid release from astrocytes prepared from human ApoE3 and ApoE4 knock-in mice.
Gong, JS; Kobayashi, M; Hayashi, H; Zou, K; Sawamura, N; Fujita, SC; Yanagisawa, K; Michikawa, M
The Journal of Biological Chemistry null
Role of low-density lipoprotein receptor in the hepatitis C virus life cycle.
Albecka, Anna, et al.
Hepatology, 55, 998-1007 (2012)
B Nan et al.
The Journal of comparative neurology, 435(1), 60-77 (2001-05-23)
Removal of the synaptic targets of olfactory receptor neurons by olfactory bulb ablation results in apoptosis of olfactory receptor neurons and up-regulation of proliferation of their progenitors. This study focuses on the expression of the neuropoietic cytokines leukemia inhibitory factor
Schwann cell nuclear remodelling and formation of nuclear and coiled bodies in Guillain-Barre syndrome.
Berciano, M T, et al.
Acta neuropathologica, 92, 386-394 (1996)
Differential regulation of amyloid-? endocytic trafficking and lysosomal degradation by apolipoprotein E isoforms.
Li, J; Kanekiyo, T; Shinohara, M; Zhang, Y; LaDu, MJ; Xu, H; Bu, G
The Journal of Biological Chemistry null

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