AB15858
Anti-Aquaporin 5 Antibody
1 mg/mL, Chemicon®
Synonym(s):
AQP5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Aqp5(11830)
rat ... Aqp5(25241)
General description
Aquaporins (AQPs) are a family of channel proteins that allow water or very small solutes to pass, functioning in tissues where the rapid and regulated transport of fluid is necessary, such as the kidney, lung, and salivary glands. Aquaporin 5 is considered to play an important role in water movement across the plasma membrane in salivary fluid secretion and corneal/lens lubrication and is a focus of studies illucidating bechanisms underlying diabetic xerostomia.
Specificity
Aquaporin 5 (AQP5). By Western blot the antibody reacts with a band at ~ 24 kDa on rat submaxillary gland lysate.
Immunogen
Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of rat Aquaporin 5.
Application
Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Aquaporin 5 in rat lung and mouse trachea tissue.
Immunohistochemistry Analysis: A representative lot from independent laboratories detected Aquaporin 5 in various paraformaldehyde fixed tissues (see references).
Immunohistochemistry Analysis: A representative lot from independent laboratories detected Aquaporin 5 in various paraformaldehyde fixed tissues (see references).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Aquaporin 5 (AQP5) Antibody is validated for use in WB for the detection of Aquaporin 5 (AQP5).
Quality
Evaluated by Western Blotting in rat salivary gland tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Aquaporin 5 in 10 µg of rat salivary gland tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Aquaporin 5 in 10 µg of rat salivary gland tissue lysate.
Target description
~28 kDa observed
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Analysis Note
Control
Tested on Rat Salivary Gland Lysate
Tested on Rat Salivary Gland Lysate
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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S Raina et al.
The Journal of biological chemistry, 270(4), 1908-1912 (1995-01-27)
The Aquaporin family of water channels plays a fundamental role in transmembrane water movements in numerous plant and animal tissues. Since the molecular pathway by which water is secreted by salivary glands is unknown, a cDNA was isolated from rat
Verification and spatial localization of aquaporin-5 in the ocular lens.
Grey, AC; Walker, KL; Petrova, RS; Han, J; Wilmarth, PA; David, LL; Donaldson, PJ; Schey, KL
Experimental Eye Research null
Wenli Yang et al.
PloS one, 9(11), e113451-e113451 (2014-11-25)
The hepatopulmonary syndrome (HPS) develops when pulmonary vasodilatation leads to abnormal gas exchange. However, in human HPS, restrictive ventilatory defects are also observed supporting that the alveolar epithelial compartment may also be affected. Alveolar type II epithelial cells (AT2) play
S M Kreda et al.
American journal of respiratory cell and molecular biology, 24(3), 224-234 (2001-03-14)
Aquaporins (AQPs) facilitate water transport across epithelia and play an important role in normal physiology and disease in the human airways. We used in situ hybridization and immunofluorescence to determine the expression and cellular localization of AQPs 5, 4, and
Yao Chen et al.
Respiratory research, 13, 1-1 (2012-01-05)
Distal alveolar morphogenesis is marked by differentiation of alveolar type (AT)-II to AT-I cells that give rise to the primary site of gas exchange, the alveolar/vascular interface. Endothelial-Monocyte Activating Polypeptide (EMAP) II, an endogenous protein with anti-angiogenic properties, profoundly disrupts
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