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411543

Sigma-Aldrich

Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate

liquid, clone HP6069, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.77

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

secondary antibodies

clone

HP6069, monoclonal

form

liquid

does not contain

preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Purified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG1 Fc fragment.
This Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG₁, Fc Fragment Specific.

Application

Enzyme Immunoassay (1:500-1:2000)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Other Notes

Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG1: 100%

Human IgG2: ≤0.1%

Human IgG3: ≤0.2%

Human IgG4: ≤0.1%

Human IgM: ≤0.1%

Human IgA: ≤0.1%

Human IgE: ≤0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG1: 100%

Human IgG2: <0.02%

Human IgG3: <0.02%

Human IgG4: <0.02%

Human IgM: <0.02%

Human IgA: <0.01%

Human IgE: <0.001%

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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F J Fasullo et al.
Clinical chemistry, 35(3), 364-368 (1989-03-01)
The objective of this study was to develop a clinical laboratory method for subclass typing of human immunoglobulin G (IgG) paraproteins. Serum proteins were isoelectrically focused (IEF) in a mini-gel and passively blotted by capillary diffusion onto untreated nitrocellulose. Unreacted
R G Hamilton
Annales de biologie clinique, 48(7), 473-477 (1990-01-01)
This study demonstrates the utility of a panel of genetically-engineered human-mouse chimeric antibodies with variable region specificity for a hapten, NP, and constant regions analogous to the different human immunoglobulin isotypes as quality control reagents. They performed well as isotype-restricted
R Jefferis et al.
Immunology letters, 10(3-4), 223-252 (1985-01-01)
Seventy-four monoclonal antibodies (McAb) of putative specificity for human IgG (11), the IgG sub-classes (59) or Gm allotypes (4) have been evaluated for reactivity and specificity in eight laboratories employing different assay techniques or protocols. For the IgG, IgG3, IgG4
R G Hamilton
Clinical chemistry, 33(10), 1707-1725 (1987-10-01)
Complement activation, cell surface-receptor binding, blocking activity, and possibly placental transfer are among the biologically important functional differences that have been detected between the four human IgG subclasses by use of polyclonal antisera. In 1985, a IUIS/WHO panel of immunologists

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