208910

Anti-Calreticulin (405-417) Rabbit pAb

liquid, Calbiochem^®

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: monkey, human, mouse, canine, hamster, bovine, rat, porcine
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Anti-Calreticulin (405-417), rabbit polyclonal, recognizes the ~63 kDa calreticulin in heat-shocked HeLa cells. It is validated for WB, ICC, IHC, and IP.

Protein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.

Recognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.

Immunogen

Human

a synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH

Application

Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)

Immunocytochemistry (see comments)

Immunoprecipitation (see comments)

Immunohistochemistry (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 50% glycerol, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Does not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Janicki, S., et al. 1997. J. Cell Biol. 139, 485.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem.270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1