17-10502
AccuChIP Trimethyl-Histone H3 Internal Control(Lys27) Chromatin Immunoprecipitation
target-specific spike-in controls that make ChIP experiments more quantitative and accurate
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About This Item
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Quality Level
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
shipped in
dry ice
Related Categories
General description
Features and Advantages
- Simple to use - just add to chromatin either before or after sonication
- Mimics the behavior of cross-linked chromatin to allow normalization of ChIP signals
- Target specific control - uses the same antibody used for your ChIP experiment
- Reduces ChIP variation due to variables such as chromatin preparation, antibody affinity, inconsistent background signals from carry over, and loss of material during handling
- Easily incorporated into virtually any ChIP protocol
- Readily detected by qPCR
- Easily incorporated into ChIP-seq libraries
- Variety of uniquely designed controls available -add multiple controls to be added to the same chromatin as an process antibody specificity
Application
Epigenetics & Nuclear Function
- Normalization of ChIP signals
- Normalization of ChIP-seq signals
- Analysis of antibody specificity
Components
AccuChIP H3K27me3 Detection Primer Mix (5 μM)
AccuChIP Dilution Buffer
Physical form
Storage and Stability
AccuChIP Spike In Reagent is provided as a 20X stock and should be diluted with the provided AccuChIP Dilution Buffer to create a working stock. Diluted AccuChIP Spike In Reagent can be stored in aliquots at -20 ºC for up to 3 months. AccuChIP Spike In reagent is composed of a peptide-double stranded DNA conjugate in a buffer containing BSA and glycerol to reduce adherence to the tube. The use of siliconized microfuge tubes for storage of diluted material is recommended.
Legal Information
Disclaimer
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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