1.50651

Purospher^® STAR RP-18 endcapped (3µm) Hibar^® HR 50-2.1

suitable for UHPLC

Properties

• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L1
• product line: Purospher^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 17% Carbon loading
• parameter: 1000 bar pressure; 65 °C max. temp.
• technique(s): LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 5 cm × 2.1 mm
• surface area: 330 m²/g
• matrix: fully porous particle
• matrix active group: C18 bonding phase
• particle size: 3 μm
• pore size: 120 Å pore size
• operating pH range: 1.5-10.5
• separation technique: reversed phase; supercritical fluid (SFC)
• storage temp.: no temp limit

Description

General description

Purospher^® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B).
These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test.

Purospher^® STAR RP-18 endcapped, 3 µm Hibar^® HR 50-2.1 is a ready to use UHPLC column suitable for connection to any UHPLC system providing a pressure stability of 1000 bar.
The column dimension of 50 mm length x 2.1 mm I.D. is the optimal choice for high throughput UHPLC separation of complex samples allowing significant solvent savings and improved sensitivity. A 2.1 mm I.D. column enables a more sustainable separations in comparison to 4, 4.6 or 3 mm I.D. column due to the use of significant less solvent for the chromatographic separation.

Learn more about Purospher^® STAR HPLC and UHPLC Columns

Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Best all-round performance according to Tanaka
• Outstanding pH stability from pH 1.5 to 10.5
• No phase collapse when using highly aqueous mobile phases
• Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (1,2-Benzanthracene): ≥ 130000
Symmetry (1,2-Benzanthracene): 0.8 - 1.4
Capacity factor (1,2-Benzanthracene): 7.0 - 10.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany