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Millipore

ReadyTube® Half Fraser Broth

For enrichment of samples for Listeria sp. and Listeria monocytogenes in accordance to ISO 11190, pkg of (225 mL in 250 mL bottle with blue screw cap and 3 loci (6 bottles per box))

Synonym(s):

Fraser Broth

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.74

Agency

ISO 11190

Quality Level

300

form

liquid (ready-to-use)

feature

ready-to-use

manufacturer/tradename

ReadyTube®

packaging

pkg of (225 mL in 250 mL bottle with blue screw cap and 3 loci (6 bottles per box))

pH

7.2 ( in H2O)

application(s)

environmental
food and beverages
microbiology

storage temp.

2-8°C

suitability

selective for Listeria spp.

Related Categories

General description

ReadyTube® is ready to use media to provide the highest level of quality and testing confidence for improving accuracy and significantly reducing the risk of false results. ReadyTube® Half Fraser broth is a selective enrichment medium designed to help recovery of Listeria sp.

Application

ReadyTube® Fraser broth is used for the primary and secondary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Analysis Note

This medium contains peptone, casein, yeast extract, and meat extract, which provide essential nutrients like carbon and nitrogenous compounds, including vitamins, amino acids, and trace ingredients. Phosphates buffer the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavine inhibit the growth of Gram-negative and Gram-positive organisms respectively, except Listeria sp. Listeria sp. hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7-Dihydroxycoumarin, a black-brown complex. Ferric ammonium citrate also enhances the growth of L. monocytogenes. The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin.

Legal Information

READYTUBE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Listeria: a Survivor

In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.

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