1.46023

HEIMPLATE Mannitol Salt Agar

Heimplate, plate diam. 90 mm, ICR Settle plate for long incubation in non-sterile pharmaceuticals -, for Staphylococcus spp.

• Synonym(s): MSA, Chapman Medium
• UNSPSC Code: 41106217
• NACRES: NA.74

Properties

• Agency: EP; JP; USP
• Quality Level: 300
• sterility: sterile
• form: plate (ready-to-use)
• feature: ready-to-use; ICR Settle plate for long incubation in non-sterile pharmaceuticals -
• packaging: pack of 20-120 plates
• manufacturer/tradename: Heimplate
• technique(s): microbiological culture: suitable
• plate diam.: 90 mm
• plate filling volume: 30 mL
• pH: 7.4 ( in H₂O)
• application(s): microbiology; pharmaceutical; surface monitoring
• storage temp.: 15-25°C
• suitability: Staphylococcus spp.

Description

General description

Mannitol Salt Agar is a modified version of the selective agar proposed by Chapman (1945) for the isolation and presumptive identification of Staphylococcus aureus. The medium supports the growth of Staphylococcus aureus, whereas its high salt content (7.5%) inhibits the growth of many other microorganisms. In contrast to most other Staphylococci, coagulase-positive S. aureus can build acids from mannitol. Therefore, they build up yellow colonies with yellow zones. Beef extract, a pancreatic digest of casein, and animal tissue in the media provide carbon, nitrogen compounds, long-chain amino acids, vitamins, trace nutrients and other essential growth factors. Phenol red indicator aids in the detection of acid produced by fermentation of mannitol.

Application

The Mannitol Salt Agar media is recommended for the isolation and presumptive identification of Staphylococcus aureus in non-sterile pharmaceuticals and foods. However, the coagulase test will need to confirm the findings.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1