06-1331

Anti-IL-23R Antibody, cytoplasmic

from rabbit, purified by affinity chromatography

• Synonym(s): interleukin 23 receptor
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_653302
• UniProt accession no.: Q5VWK5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... IL23R(149233)

Description

General description

Interleukin 23 receptor (IL-23R) is a type I cytokine receptor that is a subunit of the receptor for IL23A/IL23. IL-23R pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. IL-23R associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3.

Specificity

This antibody recognizes the cytoplasmic domain of IL-23R.
May detect other isoforms of IL-23R (see molecular weight).

Immunogen

KLH-conjugated linear peptide corresponding to human IL-23R at and around the cytoplasmic domain.

Application

Anti-IL-23R Antibody, cytoplasmic detects level of IL-23R & has been published & validated for use in WB, IH, ELISA, FC.

Does not work for mouse in flow cytometry.

Immunohistochemistry Analysis: A 1:100 dilution from a previous lot detected IL-23R in mouse spleen tissue.

Flow Cytometry Analysis: 2-10 µg from a previous lot detected IL-23R in human PBMCs. Does not work in mouse for flow cytometry.

ELISA: A previous lot of this antibody was tested on ELISA by an independent laboratory.

Quality

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected IL-23R on 10 µg of Jurkat cell lysate.

Target description

~42 kDa Observed.
May detect other isoforms of IL-23R produced by alternative splicing: isoform 1 (~72 kDa), isoform 2 (~43 kDa), isoform 3 (~41 kDa), isoform 4 (~20 kDa), isoform 5 (~30 kDa), isoform 6 (~43 kDa) and isoform 7 (~26 kDa).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable