05-949
Anti-Histidine Tagged Antibody, clone HIS.H8
clone HIS.H8, Upstate®, from mouse
Synonym(s):
HIS Tag Antibody
About This Item
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
clone
HIS.H8, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2b
shipped in
dry ice
target post-translational modification
unmodified
General description
Specificity
Immunogen
Application
2 μg/mL of a previous lot of antibody showed positive immunostaining for His tagged Akt1/PKBα in transfected HeLa cells.
Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate His Tagged proteins.
Epitope Tags & General Use
Epitope Tags
RNA Binding Protein (RBP)
Quality
Western Blot Analysis:
0.5-2 μg/mL of this lot detected His tagged Akt1/PKBα in RIPA lysates of transfected HeLa cells.
Physical form
Storage and Stability
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
RIPA lysates of transfected HeLa cells.
Other Notes
Legal Information
Disclaimer
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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