L030000

ETT Activator

0.25 M

• Synonym(s): 5-(Ethylthio)-1H-tetrazole, 5-(Ethylthio)tetrazole solution
• MDL number: MFCD00068733
• UNSPSC Code: 41116105
• PubChem Substance ID: 329817349
• NACRES: NA.21

Properties

• type: for DNA synthesis
• product line: Proligo Reagents
• storage condition: dry at room temperature
• concentration: 32-35 g/L (ETT content, titration)
• impurities: ≤30 ppm water content (Karl Fischer)
• color: clear
• SMILES string: CCSc1nnn[nH]1
• InChI: 1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)
• InChI key: GONFBOIJNUKKST-UHFFFAOYSA-N

Description

Preparation Note

Acetonitrile / 5-(Ethylthio)-1H-Tetrazole 100 / 3 (v/w)

Other Notes

Activator solution, containing 5-(Ethylthio)-1H- tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

Safety Information

• Pictograms: GHS02,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H225 - H302 + H312 + H332 - H315 - H318
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 35.6 °F
• Flash Point(C): 2.0 °C