HPLC Analysis of Proglumide Enantiomers on Astec^® CHIROBIOTIC^® V

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] 13 mM ammonium acetate; [B] acetonitrile; (90:10, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 225 nm

injection

1 μL

sample

1 mg/mL in methanol