HPLC Analysis of 3,4-Dihydroxyphenylalanine (DOPA) Enantiomers on Astec® CHIROBIOTIC® TAG
CONDITIONS
column
CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 μm particles (14024AST)
mobile phase
[A] water; [B] acetonitrile; (50:50, A:B)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 230 nm
injection
5 μL
sample
5 mg/mL in methanol
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC