A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry.

A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry.

Journal of breath research (2008-09-01)

S Smith, H Burden, R Persad, K Whittington, B de Lacy Costello, N M Ratcliffe, C S Probert

ABSTRACT

First-void urine samples were obtained from 24 elderly, asymptomatic men (median age 62.9 years). The headspace above pH adjusted urine samples were extracted using a carboxen/polydimethylsiloxane solid phase micro-extraction fibre and the volatile organic compounds analysed by gas chromatography/mass spectrometry. A total of 147 compounds were identified in the headspace of urine. The acidified samples recorded a total of 92 compounds, 27 of which were ubiquitous, basified samples 70 compounds, with 12 ubiquitous and unmodified pH samples 49, with 6 ubiquitous. Five compounds were ubiquitous irrespective of pH: acetone, methylene chloride, 4-heptanone, 2-pentanone and 2-butanone. A comparative analysis of unfrozen and frozen-thawed urine (stored at room temperature for 0, 1 and 8 h) showed that samples retained the same number of compounds although variations in the peak areas for some compounds were observed.

MATERIALS

Product Number

Brand

Product Description

57284-U

Supelco

SPME StableFlex^™ fiber assortment kit, needle size 23 ga, StableFlex fiber, for use with autosampler

57285-U

Supelco

SPME fiber assortment kit 1, for use with autosampler, needle size 23 ga

57286-U

Supelco

SPME fiber assortment kit 2, for use with autosampler, needle size 23 ga

57325-U

Supelco

SPME fiber assortment kit 4, for use with autosampler, size 24 ga

57311

Supelco

SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, needle size 24 ga, for use with autosampler

57321-U

Supelco

SPME fiber assortment kit 2, for use with autosampler, size 24 ga

20355

Supelco

ORBO^™ 78 HBr on Carboxen^® 564 specially cleaned (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

57324-U

Supelco

SPME fiber assortment kit 4, for use with manual holder, size 24 ga

57550-U

Supelco

SPME StableFlex^™ fiber assortment kit, for use with manual holder

20358

Supelco

ORBO^™ 90 Carboxen^® 564 (20/45), 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

57306

Supelco

SPME fiber assortment kit 1, for use with manual holder, needle size 24 ga

57307

Supelco

SPME fiber assortment kit 1, for use with autosampler, needle size 24 ga

57320-U

Supelco

SPME fiber assortment kit 2, for use with manual holder, needle size 24 ga

57551-U

Supelco

SPME StableFlex^™ fiber assortment kit, needle size 24 ga, for use with autosampler

57287-U

Supelco

SPME fiber assortment kit 4, for use with autosampler, needle size 23 ga

29534-U

Supelco

Carboxen^® 569, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea

68554

Supelco

γ-Hexalactone, analytical standard

57362-U

Supelco

SPME fiber assortment kit 5, needle size 23 ga, for use with autosampler

28718-U

Supelco

Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

57902-U

Supelco

SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga, metal alloy fiber

57928-U

Supelco

SPME fiber assembly Polydimethylsiloxane (PDMS), metal alloy fiber, d_f 100 μm(PDMS, needle size 23 ga, for use with autosampler

54041-U

Supelco

Carboxen^® 1000 Reversible SPE Tube, 200 mg/mL, pk 30

57439-U

Supelco

SPME-OC Fiber Assembly, Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 75 μm(Coating thickness includes 65 μm coating + 10 μm OC (overcoating), needle size 23 ga, pkg of 3 ea, fiber L 1 cm

57914-U

Supelco

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 2 cm

57912-U

Supelco

SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, metal alloy fiber, for use with autosampler, fiber L 1 cm, pkg of 1 × ea

57906-U

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 85 μm(CAR/PDMS, for use with autosampler, needle size 23 ga, metal alloy fiber