GE28-4082-61
HiTrap® MabSelect™ Xtra
Cytiva 28-4082-61, pack of 5 × 5 mL
About This Item
Recommended Products
ligand
recombinant protein A (E. coli)
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-4082-61
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked agarose
avg. part. size
75 (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-12(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
Related Categories
General description
Application
Features and Benefits
- Increased dynamic binding capacity with smaller particle size and greater porosity than MabSelect™ medium.
- MabSelect™ Xtra uses the same recombinant Protein A ligand as MabSelect™
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
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