E1411

Proline Specific Endopeptidase from Flavobacterium sp.

lyophilized powder, ≥5.0 units/mg solid

• Synonym(s): EndoProC, Endoprolylpeptidase, Endoproteinase Pro-C, PEPase, Prolyl Endopeptidase
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Flavobacterium spp.)
• form: lyophilized powder
• specific activity: ≥5.0 units/mg solid
• mol wt: ~78 kDa
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Proline specific endopeptidase is useful for the determination of amino acid sequences of peptides and proteins containing proline residues. Proline specific endopeptidases may be useful in cancer, neurology and diabetes research . Prolyl endopeptidase (PEP) may be used as a drug target for neuropsychiatric diseases such as stress disorder, depression, and schizophrenia .

Biochem/physiol Actions

An endopeptidase is a proteolytic peptidase that breaks peptide bonds of nonterminal amino acids. Product E1411 is specific for proline (Pro). The Prolyl endopeptidase (PEP) mechanism is thought to be an induced fit mechanism, where the native enzyme exists in a conformationally flexible opened state but is changed to a closed state upon substrate binding .

Unit Definition

One unit causes the formation of one micromole of p-nitroaniline per minute under specific pH and temperature conditions.

Physical form

White amorphous lyophilized powder

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable