E6758

Ethylenediaminetetraacetic acid

anhydrous, crystalline, BioReagent, suitable for cell culture

• Synonym(s): (Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid
• Linear Formula: (HO₂CCH₂)₂NCH₂CH₂N(CH₂CO₂H)₂
• CAS Number: 60-00-4
• Molecular Weight: 292.24
• Beilstein: 1716295
• EC Number: 200-449-4
• MDL number: MFCD00003541
• UNSPSC Code: 12161700
• eCl@ss: 39030907
• PubChem Substance ID: 24894634
• NACRES: NA.25

Properties

• grade: anhydrous
• product line: BioReagent
• Assay: ≥98.5%
• form: crystalline
• reaction suitability: reagent type: chelator
• technique(s): cell culture | mammalian: suitable
• pH: 2.5 (23 °C, 10 g/L)
• mp: 250 °C (dec.) (lit.)
• solubility: 3 M NaOH: 100 mg/mL
• density: 1.46 g/cm³ at 20 °C
• cation traces: C: 40.7-41.5%; N: 9.3-9.8%
• SMILES string: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
• InChI key: KCXVZYZYPLLWCC-UHFFFAOYSA-N

Description

Application

Ethylenediaminetetraacetic acid has been used:

• as a component of peptide dilution buffer for intracellular BH3 (Bcl-2 homology (BH) domain) profiling and validation
• as a medium component in the hepatic stellate cell (HSCs) culture
• as a component of homogenization buffer for protein extraction from eye tissues
• for stopping benzonase reaction in lysis buffer

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood samples. EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia. EDTA along with tetracycline imparts serves as root conditioning agent and provides relief in gum infection. EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis. Calcium Disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves