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CMC0006

Sigma-Aldrich

SIG10 ULTRA Electrocompetent Cells

for BAC & cosmid cloning

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

biological source

Escherichia coli

grade

for molecular biology

form

buffered aqueous solution

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥4 x 1010 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The SIG10 ULTRA Electrocompetent Cells have the highest transformation efficiency available. They are to be used for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

  • share the most useful genetic elements of standard cloning strains like DH5α DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
  • provide the performance researchers need with ease of use.
  • provide solutions for a wide range of applications at economical prices.
  • Blue - white screening

Components

  • SIG10 ULTRA electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning

Legal Information

DH5α is a trademark of Invitrogen Corp.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids


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