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CMC0003

Sigma-Aldrich

SIG10 HIGH Electrocompetent Cells

for protein expression and DNA plasmid production

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

biological source

Escherichia coli

grade

for molecular biology

form

buffered aqueous solution

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The SIG10 HIGH Electrocompetent Cells are high efficiency cells with a substantially lower cost per reaction and are the most economical choice for standard cloning and library construction.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

  • share the most useful genetic elements of standard cloning strains like DH5a, DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
  • incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability.
  • provide solutions for a wide range of applications at economical prices.
  • Blue - white screening

Components

  • SIG10 HIGH electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning

Storage Class Code

10 - Combustible liquids


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