P8340
Protease Inhibitor Cocktail
for use with mammalian cell and tissue extracts, DMSO solution
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General description
Protease Inhibitor Cocktail for mammalian cell and tissue extracts, in DMSO solution. It contains six individual components for inhibiting serine, cysteine, and acid proteases, and aminopeptidases.
Specificity
Inhibits serine, cysteine, and acid proteases, and aminopeptidases.
Application
Ideal for use as a buffer component during sonication, lysis buffer, and RIPA buffer
Provides protection against endogenous enzyme activity in cell and tissue extracts
Provides protection against endogenous enzyme activity in cell and tissue extracts
Biochem/physiol Actions
This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases.
Features and Benefits
Broad-spectrum inhibition: inhibits multiple classes of proteases and aminopeptidases
Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C
Consistent performance: ready-to-use solution
Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C
Consistent performance: ready-to-use solution
Components
AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
Caution
This cocktail should be stored at -20 °C.
Preparation Note
One mL of this cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g of bovine liver or 10 mL of lysate from CHO cells. This product is supplied as a solution in DMSO.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Certificates of Analysis (COA)
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